User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/03/07

From OpenWetWare

< User:David Dreher | Notebook | Chromatin controlled cell pattern | 2013 | 03(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/03/07 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
Current revision (00:01, 19 March 2013) (view source)
(Entry title)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==03/07/13==
-
* Insert content here...
+
HEK293 Luc #4 fixed DAPI staining
 +
 
 +
----
 +
* DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O
 +
* Diluted Hoescht to working concentration (1x) in 1xPBS.  
 +
* Fixing cells with formaldehyde
 +
* Adding Hoescht to the cells, let sit in for 10 mins in the dark to develop signal
 +
 
 +
----
 +
Results:
 +
* Good DAPI signal
 +
* Detection Algorithm failed due to very high cell density
 +
 
 +
----
 +
Next steps:
 +
* Growing and imaging cells in less dense conditions

Current revision

Project name Main project page
Previous entry      Next entry

03/07/13

HEK293 Luc #4 fixed DAPI staining


  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Fixing cells with formaldehyde
  • Adding Hoescht to the cells, let sit in for 10 mins in the dark to develop signal

Results:

  • Good DAPI signal
  • Detection Algorithm failed due to very high cell density

Next steps:

  • Growing and imaging cells in less dense conditions



Personal tools