User:David J. Klinke II

David J. Klinke II

Assistant Professor
Department of Chemical Engineering
Department of Microbiology, Immunology, and Cell Biology
West Virginia University
P.O. Box 6102
Morgantown, WV 26506-6102

david.klinke at mail.wvu.edu

Education and Industrial Experience

• 1999-2005, Biosystems Engineer, Entelos, Inc., Foster City, CA
• 1999, Post-Doctoral Fellow, Sandia National Laboratory, Livermore, CA
• 1998, PhD, Northwestern University, Evanston IL
• 1995, MS, Northwestern University, Evanston IL
• 1992, BS, Virginia Tech, Blacksburg, VA

Research interests

• Cellular Signal Transduction in Cancer and Immunology
• Proteomics and Flow Cytometry
• Bayesian statistics

Publications

1. Klinke DJ 2nd and Brundage KM. Scalable analysis of flow cytometry data using R/Bioconductor. Cytometry A. 2009 Aug;75(8):699-706. DOI:10.1002/cyto.a.20746 |

   Provides a tutorial for using R/Bioconductor to process flow cytometry data.

2. Klinke DJ 2nd. Validating a dimensionless number for glucose homeostasis in humans. Ann Biomed Eng. 2009 Sep;37(9):1886-96. DOI:10.1007/s10439-009-9733-y |

   Develops and validates a dimensionless number for the ratio of insulin production and insulin-dependent glucose metabolism in humans.

3. Klinke DJ 2nd, Ustyugova IV, Brundage KM, and Barnett JB. Modulating temporal control of NF-kappaB activation: implications for therapeutic and assay selection. Biophys J. 2008 Jun;94(11):4249-59. DOI:10.1529/biophysj.107.120451 |

   Finds that our ability to observe significant biological events is limited by the signal-to-noise characteristics of the assays that we use to observe cellular responses.

4. Klinke DJ 2nd. Extent of beta cell destruction is important but insufficient to predict the onset of type 1 diabetes mellitus. PLoS One. 2008 Jan 2;3(1):e1374. DOI:10.1371/journal.pone.0001374 |

   Evaluates the conventional wisdom regarding the extent of beta cell destruction required before presenting with symptoms of type 1 diabetes.

5. Klinke DJ 2nd. Integrating epidemiological data into a mechanistic model of type 2 diabetes: validating the prevalence of virtual patients. Ann Biomed Eng. 2008 Feb;36(2):321-34. DOI:10.1007/s10439-007-9410-y |

   Provides a glimpse of the kind of models developed by Entelos, Inc.

6. Klinke DJ 2nd. A multi-scale model of dendritic cell education and trafficking in the lung: implications for T cell polarization. Ann Biomed Eng. 2007 Jun;35(6):937-55. DOI:10.1007/s10439-007-9318-6 |
7. Klinke DJ 2nd. An age-structured model of dendritic cell trafficking in the lung. Am J Physiol Lung Cell Mol Physiol. 2006 Nov;291(5):L1038-49. DOI:10.1152/ajplung.00048.2006 |
8. Klinke DJ 2nd. The ratio of P40 monomer to dimer is an important determinant of IL-12 bioactivity. J Theor Biol. 2006 May 21;240(2):323-35. DOI:10.1016/j.jtbi.2005.09.022 |

Awards

• 1992 Summa cum laude alumnus, Commonweath Scholar, Virginia Tech
  
• 2008 - 2009 Research Starter Grant in Informatics
  

Useful links

• Introductory tutorial
• OpenWetWare help pages