User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/04/25: Difference between revisions

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==Remains of the Day==
==Remains of the Day==
* Check cloning plates and do colony PCR/RFLP screen of positives.  
* Check cloning plates and do colony PCR/RFLP screen of positives using M13 primers.  
* Photograph and extract rhizospheres and roots from more plants downstairs.  
* Photograph and extract rhizospheres and roots from more plants downstairs.  
* Extract DNA from samples acquired yesterday and today.
* Extract DNA from samples acquired yesterday and today.
{| border="1" cellpadding="21" align="center"
|-
! width="50"|Soil Treatment|| width="50"|Genotype || width="50"|Shoot Weight || width="50"|Root Weight
|-
| Untreated TVD Soil || Heinz 2401 || 6.91||  3.23
|-
| Untreated TVD Soil || Heinz 2401 || 4.88||  3.30
|-
| Untreated TVD Soil || Heinz 2401 || 5.08||  2.16
|-
| Untreated TVD Soil || Heinz 2401 || 5.05||  2.46
|-
| Untreated TVD Soil || Heinz 2401 || 4.57||  3.18
|-
| Stenotrophomonas spp. 3-14 Innoculated TVD Soil || Heinz 2401 || 5.28||  3.90
|-
| Stenotrophomonas spp.3-14 Innoculated TVD Soil || Heinz 2401 || 5.85||  1.79
|-
| Stenotrophomonas spp. 3-14 Innoculated TVD Soil || Heinz 2401 || 7.02||  3.39
|-
| Stenotrophomonas spp. 3-14 Innoculated TVD Soil || Heinz 2401 || 5.17||  2.54
|-
| Stenotrophomonas spp. 3-14 Innoculated TVD Soil || Heinz 2401 || 5.15||  2.46
|-
| TBG Liquid 1% TVD Soil || Heinz 2401 || 6.09||  2.21
|-
| TBG Liquid 1% TVD Soil || Heinz 2401 || 6.88||  1.68
|-
| TBG Liquid 1% TVD Soil || Heinz 2401 || 3.83||  0.79
|-
| TBG Liquid 1% TVD Soil || Heinz 2401 || 2.59||  0.88
|-
| TBG Liquid 1% TVD Soil || Heinz 2401 || 4.75||  0.97
|-
| TBG Solid TVD Soil || Heinz 2401 || 3.64||  1.39
|-
| TBG Solid TVD Soil || Heinz 2401 || 3.20||  1.06
|-
| TBG Solid  TVD Soil || Heinz 2401 || 3.73||  1.48
|-
| TBG Solid  TVD Soil || Heinz 2401 || 3.15||  1.51
|-
| TBG Solid  TVD Soil || Heinz 2401 || 3.61||  1.44
|-
|}




Revision as of 11:25, 24 April 2013

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Remains of the Day

  • Check cloning plates and do colony PCR/RFLP screen of positives using M13 primers.
  • Photograph and extract rhizospheres and roots from more plants downstairs.
  • Extract DNA from samples acquired yesterday and today.


Soil Treatment Genotype Shoot Weight Root Weight
Untreated TVD Soil Heinz 2401 6.91 3.23
Untreated TVD Soil Heinz 2401 4.88 3.30
Untreated TVD Soil Heinz 2401 5.08 2.16
Untreated TVD Soil Heinz 2401 5.05 2.46
Untreated TVD Soil Heinz 2401 4.57 3.18
Stenotrophomonas spp. 3-14 Innoculated TVD Soil Heinz 2401 5.28 3.90
Stenotrophomonas spp.3-14 Innoculated TVD Soil Heinz 2401 5.85 1.79
Stenotrophomonas spp. 3-14 Innoculated TVD Soil Heinz 2401 7.02 3.39
Stenotrophomonas spp. 3-14 Innoculated TVD Soil Heinz 2401 5.17 2.54
Stenotrophomonas spp. 3-14 Innoculated TVD Soil Heinz 2401 5.15 2.46
TBG Liquid 1% TVD Soil Heinz 2401 6.09 2.21
TBG Liquid 1% TVD Soil Heinz 2401 6.88 1.68
TBG Liquid 1% TVD Soil Heinz 2401 3.83 0.79
TBG Liquid 1% TVD Soil Heinz 2401 2.59 0.88
TBG Liquid 1% TVD Soil Heinz 2401 4.75 0.97
TBG Solid TVD Soil Heinz 2401 3.64 1.39
TBG Solid TVD Soil Heinz 2401 3.20 1.06
TBG Solid TVD Soil Heinz 2401 3.73 1.48
TBG Solid TVD Soil Heinz 2401 3.15 1.51
TBG Solid TVD Soil Heinz 2401 3.61 1.44



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