User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/14: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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==Remains of the Day==
==Remains of the Day==
* First thing, start HhaI and DdeI digests of plate 1 and plate 2 of Mt. Brydges Maize experiment. For Tilbury grafted plants and organic amendmant bulk soil, use HhaI and BstNI
* First thing, start HhaI and DdeI digests of plate 1 and plate 2 of Mt. Brydges Maize experiment. For Tilbury grafted plants and organic amendmant bulk soil, use HhaI and BstNI. Submit TRFLP samples (about 576 in total!) - didn't time it right, so I'll have to send the samples in tommorow instead.
* George encouraged me to develop the NifH TRFLP, in addition to one for several antibiotic genes, and phosphate solubilizing genes. He refered me to his previous report (called, "HAL final report") for primer information, etc.
* I started trying to write the Terrabiogen report.
* George also wants to bioassay the soil being sent up from California (round 2 of the strawberry project). I'm going to try to find some inbred strawberry seed to use in the bioassay. There's one diploid variety with a genome project going on, so I'll order some of that from the USDA (http://www.nature.com/ng/journal/v43/n2/full/ng.740.html) and some other varieties like Allstar (popular all over Michigan and US), Honeoye (most popular/productive strawberry but highly susceptible to Verticillium wilt), Veestar (popular for pick your own in Ontario, but susceptible to Verticillium), and the California variety, Seascape (heat tolerant, virus tolerant)




Latest revision as of 22:15, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • First thing, start HhaI and DdeI digests of plate 1 and plate 2 of Mt. Brydges Maize experiment. For Tilbury grafted plants and organic amendmant bulk soil, use HhaI and BstNI. Submit TRFLP samples (about 576 in total!) - didn't time it right, so I'll have to send the samples in tommorow instead.
  • George encouraged me to develop the NifH TRFLP, in addition to one for several antibiotic genes, and phosphate solubilizing genes. He refered me to his previous report (called, "HAL final report") for primer information, etc.
  • I started trying to write the Terrabiogen report.
  • George also wants to bioassay the soil being sent up from California (round 2 of the strawberry project). I'm going to try to find some inbred strawberry seed to use in the bioassay. There's one diploid variety with a genome project going on, so I'll order some of that from the USDA (http://www.nature.com/ng/journal/v43/n2/full/ng.740.html) and some other varieties like Allstar (popular all over Michigan and US), Honeoye (most popular/productive strawberry but highly susceptible to Verticillium wilt), Veestar (popular for pick your own in Ontario, but susceptible to Verticillium), and the California variety, Seascape (heat tolerant, virus tolerant)



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