User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway. | * Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway. | ||
* After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction | * After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction | ||
* Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of | * Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the into semi-solid LGI (50 ul of 10X diluted stuff for shoot, 50 ul of 100X diluted stuff for roots). | ||
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Latest revision as of 22:17, 26 September 2017
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Remains of the Day
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