User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/21

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(Remains of the Day)
Current revision (19:07, 21 February 2013) (view source)
(Remains of the Day)
 

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Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Bacterial PCR of the root samples from TVD growth room rep 2 failed again - just gonna go ahead and use the stuff from the first round and accept that they failed
  • Did restriction of all the bacterial (HhaI) and fungal (BstNI) PCR samples for TRFLP. Did not purify the reactions.
  • In the morning, I put tissue cultured Kennebec potatoes into 1:100, 1:250, and 1:500 dilutions (1 mL of which was added to 9 mL of PNCM, then autoclaved) of TBG liquid and FX fraction. Wait for a month to assay growth responses with photos and weights.

image:Feb21,2013Gel.tif



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