User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/03/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Did the rest of the potato endophyte inoculations today - 6H -> 4D (or so).
* Started the physical TRFLP on pooled PCRs (of the 5 rep 1 Heinz 2401 rhizospheres from the growth room and the 5 washed roots from Ilbury 2012). Got as far as the restriction step and leaving them digesting overnight at room temp (25) on my desk
* Recieved tomato rhizosphere samples from Saskatoon. 5 of each - plants from field 1, 7, 8, 9G, and 9B. Did fungal TRFLP PCRs on them, but screwed up, potentially missing all of 8 or 9B.  




Latest revision as of 22:34, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Did the rest of the potato endophyte inoculations today - 6H -> 4D (or so).
  • Started the physical TRFLP on pooled PCRs (of the 5 rep 1 Heinz 2401 rhizospheres from the growth room and the 5 washed roots from Ilbury 2012). Got as far as the restriction step and leaving them digesting overnight at room temp (25) on my desk
  • Recieved tomato rhizosphere samples from Saskatoon. 5 of each - plants from field 1, 7, 8, 9G, and 9B. Did fungal TRFLP PCRs on them, but screwed up, potentially missing all of 8 or 9B.



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