User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/03

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(Autocreate 2013/04/03 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
Current revision (17:54, 3 April 2013) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Write up your awesome plans and results here...
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* Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
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* Cut potatoes for innoculation with the rest of the endophytes tommorow.
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* Found good article about induction of SAR with innoculation with Methylobacteria (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046802#pone.0046802-Rosenblueth1)
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[[image:April 3, 2013Gel.tif]]
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Remains of the Day

  • Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
  • Cut potatoes for innoculation with the rest of the endophytes tommorow.

image:April 3, 2013Gel.tif


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