User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Remains of the Day==
==Remains of the Day==
* Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 primers.  
* Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
 
* Cut potatoes for innoculation with the rest of the endophytes tommorow.
 
* Found good article about induction of SAR with innoculation with Methylobacteria (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046802#pone.0046802-Rosenblueth1)


[[image:April 3, 2013Gel.tif]]
[[image:April 3, 2013Gel.tif]]

Revision as of 15:54, 3 April 2013

Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Remains of the Day

  • Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
  • Cut potatoes for innoculation with the rest of the endophytes tommorow.