User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/08: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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* Check for Silwet L-77 and order if they don't have
* Check for Silwet L-77 and order if they don't have
* Grow all 8 sets of bacterial spot and speck and take CFU measurements at 3 different OD600 each to estimate bacterial populations
* Grow all 8 sets of bacterial spot and speck and take CFU measurements at 3 different OD600 each to estimate bacterial populations
* Work on tomato paper (figures)
* Only two of my 32 sequences were actually processed (they were thrown out since the water evaporated) so I did 32 more colony PCRs today (40 cycles, 52 degree annealing)




Latest revision as of 22:37, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Help Steph with bacteria for strawberry project (but let her do it)
  • Check for Silwet L-77 and order if they don't have
  • Grow all 8 sets of bacterial spot and speck and take CFU measurements at 3 different OD600 each to estimate bacterial populations
  • Work on tomato paper (figures)
  • Only two of my 32 sequences were actually processed (they were thrown out since the water evaporated) so I did 32 more colony PCRs today (40 cycles, 52 degree annealing)



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