User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/05/03: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEM"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Stephanie finished extraction of rhizosphere DNA from trial 2 of the strawberry experiment (Albion speedlings grown in growth room with diffferent washes and bacterial innoculations)
* Growing 2 L of the Pseudomonas for tomatoes for George (at room temp with gentle shaking over the weekend)
* Mixed 1 ml of the below overnights in R2A with 1 mL of water with 0.2% silwett and am inoculating 20 ul onto tomato leaves since no other symptoms appear to be coming (put these tubes into the 4 degree and innoculated on Sunday - 20 ul per leaf with a mix up and inoculation of Pst T6D1 onto the Pst DC01 treatment and no inoculation of the Pst T6D1 treatment (so this treatment was only inoculated the one time like two weeks ago))
 
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| width="50"|Disease caused || width="50"|Species/Strain || width="50"|OD600
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| Bacterial speck || P. syringae DC01 || 1.32||
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| Bacterial speck  || P. syringae DC08|| 1.18||
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| Bacterial speck  || P. syringae DC95|| 0.86||
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| Bacterial speck || P. syringae T6D1|| 1.25||
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| Bacterial spot  || X. gardneri 444|| 1.07||
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| Bacterial spot || X. gardneri DC00|| NA||
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| Bacterial spot || X. gardneri DC03|| NA ||
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| Bacterial spot || X. gardneri 1B5B|| 1.51||
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Latest revision as of 22:40, 26 September 2017

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Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Stephanie finished extraction of rhizosphere DNA from trial 2 of the strawberry experiment (Albion speedlings grown in growth room with diffferent washes and bacterial innoculations)
  • Growing 2 L of the Pseudomonas for tomatoes for George (at room temp with gentle shaking over the weekend)
  • Mixed 1 ml of the below overnights in R2A with 1 mL of water with 0.2% silwett and am inoculating 20 ul onto tomato leaves since no other symptoms appear to be coming (put these tubes into the 4 degree and innoculated on Sunday - 20 ul per leaf with a mix up and inoculation of Pst T6D1 onto the Pst DC01 treatment and no inoculation of the Pst T6D1 treatment (so this treatment was only inoculated the one time like two weeks ago))
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Disease caused Species/Strain OD600
Bacterial speck P. syringae DC01 1.32
Bacterial speck P. syringae DC08 1.18
Bacterial speck P. syringae DC95 0.86
Bacterial speck P. syringae T6D1 1.25
Bacterial spot X. gardneri 444 1.07
Bacterial spot X. gardneri DC00 NA
Bacterial spot X. gardneri DC03 NA
Bacterial spot X. gardneri 1B5B 1.51


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