User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/06/20: Difference between revisions
(Autocreate 2013/06/20 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers) |
|||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Remains of the Day== | ==Remains of the Day== | ||
* | * Gonna combine optimized Oomycete DC6 primer tagged with 6FAM (OomF DavDC6 – GAGGGACTTTtGGGTAATCA) with OomR for about 1600 bp TRFLP amplicons and then use the below primers for nested detection of particular species. | ||
(Actually ordered Pythium irregulare: PythIrreg Fwd – CGGAGCGGCGGGTGCTGTK with PythIrreg Rev – CaCCGAagtcgCcgACAG) [adapted from, “Species-specific PCR primers for Pythium developed from ribosomal ITS1 region”] | |||
(Actually ordered Pythium ultimum: PythUltim Fwd – ACGAAGGTTGGTCTGTTG with PythUltim Rev – TCTCTACGCAACTAAATGC) [adapted from, “DETECTION OF PYTHIUM ULTIMUM FROM SOIL USING PCR MICROPLATE HYBRIDIZATION WITH SPECIES-SPECIFIC PRIMERS”] | |||
(Actually ordered Phytophthora fragariae: PhytopFrag Fwd– with cACTTAGTTGGGGGCCTGTC with PhytopFrag Rev – CGCCGACTGGCCACACAG) [adapted from, “Validation and Exploitation of a PCR-based Diagnostic for Detecting Phytophthora in Strawberry and Raspberry”] | |||
(Actually ordered Phytophthora infestans: PhytopInfest Fwd – TGGGCGAGCCCTATCAAAA with PhytopInfest Rev – CGATTCAAATGCCAAGCTA)[adapted from, “Development of a species-specific and sensitive detectionassay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil”] | |||
Also gonna order fresh 27F Degen with a 6FAM label on it: AGRRTTYGATYHTGGYTYAG (noted that I put a typo into the sequence I used in my PhD) | |||
Revision as of 13:27, 20 June 2013
 Applied Soil Microbial Ecology
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Remains of the Day
(Actually ordered Pythium irregulare: PythIrreg Fwd – CGGAGCGGCGGGTGCTGTK with PythIrreg Rev – CaCCGAagtcgCcgACAG) [adapted from, “Species-specific PCR primers for Pythium developed from ribosomal ITS1 region”] (Actually ordered Pythium ultimum: PythUltim Fwd – ACGAAGGTTGGTCTGTTG with PythUltim Rev – TCTCTACGCAACTAAATGC) [adapted from, “DETECTION OF PYTHIUM ULTIMUM FROM SOIL USING PCR MICROPLATE HYBRIDIZATION WITH SPECIES-SPECIFIC PRIMERS”] (Actually ordered Phytophthora fragariae: PhytopFrag Fwd– with cACTTAGTTGGGGGCCTGTC with PhytopFrag Rev – CGCCGACTGGCCACACAG) [adapted from, “Validation and Exploitation of a PCR-based Diagnostic for Detecting Phytophthora in Strawberry and Raspberry”] (Actually ordered Phytophthora infestans: PhytopInfest Fwd – TGGGCGAGCCCTATCAAAA with PhytopInfest Rev – CGATTCAAATGCCAAGCTA)[adapted from, “Development of a species-specific and sensitive detectionassay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil”] Also gonna order fresh 27F Degen with a 6FAM label on it: AGRRTTYGATYHTGGYTYAG (noted that I put a typo into the sequence I used in my PhD)
| |
