User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/07/30

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(Autocreate 2013/07/30 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
Current revision (17:53, 30 July 2013) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Write up your awesome plans and results here...
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* Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
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* Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.  

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Remains of the Day

  • Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
  • Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.



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