User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/07/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Remains of the Day==
==Remains of the Day==
* Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
* Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
* Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time.  
* Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.  




Latest revision as of 23:08, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
  • Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.



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