User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/07

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(Remains of the Day)
(Remains of the Day)
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* Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes)
* Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes)
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html)  -  (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg)  -  (http://www.ncbi.nlm.nih.gov/pubmed/19554039)
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html)  -  (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg)  -  (http://www.ncbi.nlm.nih.gov/pubmed/19554039)
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** Thinking on it more, it seems likely that real time PCR would be better. For P. fluorescencs this may be a start (http://aem.asm.org/content/72/4/2421.full), for B. subtilis ( )
* Write up tree stuff to include in Kristin report
* Write up tree stuff to include in Kristin report

Revision as of 12:18, 7 October 2013

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