User:David K. Barclay/Notebook/Chromatin State Sensor/2015/04/17: Difference between revisions

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==Entry title==
==U2OS Cell Split==
* Insert content here...
* Splitting U2OS cells from thawed passage. Obtained from Ben.
* McCoy's 5A media, PBS and trypsin heated to 37C
* U2OS cells at 90% confluence with T-75 flask at time of split. Growing in culture since 4/14 (last split).
 
* Passages:
# 2- T-75 Flasks (1-9)
# 1- 6 Well Plate (1-3)
 
 




Revision as of 12:00, 17 April 2015

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U2OS Cell Split

  • Splitting U2OS cells from thawed passage. Obtained from Ben.
  • McCoy's 5A media, PBS and trypsin heated to 37C
  • U2OS cells at 90% confluence with T-75 flask at time of split. Growing in culture since 4/14 (last split).
  • Passages:
  1. 2- T-75 Flasks (1-9)
  2. 1- 6 Well Plate (1-3)




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