User:David K. Barclay/Notebook/Chromatin State Sensor/2015/06/24: Difference between revisions
From OpenWetWare
(Autocreate 2015/06/24 Entry for User:David_K._Barclay/Notebook/Chromatin_State_Sensor) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==David Barclay - 06/24/15== | ||
* | |||
* Thawing - TC1alpha, split 2:8 | |||
* Passaging- U2OS 1:9 | |||
---- | |||
'''Passaging - U2OS'' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells] | |||
* Cell culture medium is: McCoy's 5A, 10% FBS (tet-free), 1% P/S | |||
* Use 90-100% confluent '''T-25''' | |||
* Make 1 new T-75 at a 1:10 dilution | |||
* Passage number: 0 | |||
---- | |||
'''Thawing''' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:ThawingCells Thawing Cells] | |||
* Cell culture medium is: DMEM, 20% FBS (tet-free), 1% P/S | |||
* From Frozen Cell Stocks (3 remaining) | |||
* Make 1 new T-25 | |||
* Passage number: alpha | |||
Revision as of 15:59, 24 June 2015
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
David Barclay - 06/24/15
'Passaging - U2OS
Thawing
| |
