User:David K. Barclay/Notebook/Chromatin State Sensor/2015/06/24: Difference between revisions

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'''Passaging - U2OS''
'''Passaging - U2OS'''


* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells]
* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells]

Revision as of 15:59, 24 June 2015

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David Barclay - 06/24/15

  • Thawing - TC1alpha, split 2:8
  • Passaging- U2OS 1:9

Passaging - U2OS

  • Protocol: Splitting Cells
  • Cell culture medium is: McCoy's 5A, 10% FBS (tet-free), 1% P/S
  • Use 90-100% confluent T-25
  • Make 1 new T-75 at a 1:10 dilution
  • Passage number: 0


Thawing

  • Protocol: Thawing Cells
  • Cell culture medium is: DMEM, 20% FBS (tet-free), 1% P/S
  • From Frozen Cell Stocks (3 remaining)
  • Make 1 new T-25
  • Passage number: alpha



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