User:David K. Barclay/Notebook/Chromatin State Sensor/2015/08/23: Difference between revisions
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== | ==David Barclay 08/23== | ||
'''Passaging - U20S''' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells] | |||
* Cell culture medium is: McCoys 5A, 10% FBS (tet-free), 1% P/S | |||
* Use 90-100% confluent '''T-75''' | |||
* Make 1 new T-75 at 10% | |||
* Make 1 new 6 well @ 50% (1.5mL into 2.0mL total volume) | |||
* Passage number: 4 | |||
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Revision as of 17:01, 23 August 2015
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David Barclay 08/23Passaging - U20S
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