User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/04/14: Difference between revisions
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(Autocreate 2014/04/14 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors) |
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== | ==RNA extraction== | ||
* | * RNeasy spin column after TRIzol prep | ||
* See following protocol: http://openwetware.org/wiki/Haynes:TRIzol_RNeasy | |||
==Set up== | |||
*TRIzol added to treated/untreated cell pair at time points | |||
{|3T || 3 day treated | |||
|- | |||
| 3U || 3 day untreated | |||
|- | |||
| 6T || 6 day treated | |||
|- | |||
| 6U || 6 day untreated | |||
|- | |||
| 10T || 10 day treated | |||
|- | |||
| 10U || 10 day untreated | |||
|} | |||
Revision as of 13:33, 14 April 2014
 Cell Fate Switch by Synthetic Transcription Factors
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RNA extraction
Set up
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