User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/04/14

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(Autocreate 2014/04/14 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors)
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==Entry title==
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==RNA extraction==
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* Insert content here...
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* RNeasy spin column after TRIzol prep
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* See following protocol: http://openwetware.org/wiki/Haynes:TRIzol_RNeasy
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==Set up==
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*TRIzol added to treated/untreated cell pair at time points
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{|3T || 3 day treated
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|-
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| 3U || 3 day untreated
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|-
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| 6T || 6 day treated
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|-
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| 6U || 6 day untreated
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|-
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| 10T || 10 day treated
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|-
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| 10U || 10 day untreated
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|}
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Revision as of 16:33, 14 April 2014

Cell Fate Switch by Synthetic Transcription Factors Main project page
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RNA extraction

Set up

  • TRIzol added to treated/untreated cell pair at time points
3U 3 day untreated
6T 6 day treated
6U 6 day untreated
10T 10 day treated
10U 10 day untreated




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