User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/06/25: Difference between revisions
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* [http://openwetware.org/wiki/Haynes:SYBRGreen Standard Procedure: SYBRGreen Assay] | * [http://openwetware.org/wiki/Haynes:SYBRGreen Standard Procedure: SYBRGreen Assay] | ||
===Target Genes=== | |||
{| {{table}} | {| {{table}} | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
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|- | |- | ||
| nTc || --- || A,B,C,D,E,F,G,H8-10 || --- | | nTc || --- || A,B,C,D,E,F,G,H8-10 || --- | ||
|} | |||
===Results=== | |||
{| {{table}} | |||
|- bgcolor=#cfcfcf | |||
| Gene || dilution || +/- PcTF || Mean CP || delta CP || double delta CP | |||
|- | |||
| PDX1 || 1:10 || treated || 0 || 0 || Invalid | |||
|- | |||
| PDX1 || 1:10 || untreated || 0 || 0 || 0 | |||
|- | |||
| MAFA || 1:10 || treated || 34.440 || 0.3350 || 0.3624 | |||
|- | |||
| MAFA || 1:10 || untreated || 34.936 || 0.9245 || 1 | |||
|- | |||
| GLP1R || 1:10 || treated || 40 || 7.10E-3 || 0.2568 | |||
|- | |||
| GLP1R || 1:10 || untreated || 40 || 2.76E-2 || 1 | |||
|- | |||
| PCSK1 || 1:10 || treated || 0 || Invalid || Invalid | |||
|- | |||
| PCSK1 || 1:10 || untreated || 0 || Invalid || Invalid | |||
|- | |||
| IAPP || 1:10 || treated || 38.363 || 2.21E-2 || 3.14E-2 | |||
|- | |||
| IAPP || 1:10 || untreated || 35.333 || 0.7022 || 1 | |||
|- | |||
| KCNQ2 || 1:10 || treated || 31.621 || 2.363 || 0.5395 | |||
|- | |||
| KCNQ2 || 1:10 || untreated || 32.6919 || 4.381 || 1 | |||
|- | |||
| mCh || 1:1000 || treated || 33.3063 || 0.7350 || Invalid | |||
|- | |||
| mCh || 1:1000 || untreated || 0 || 0 || Invalid | |||
|- | |||
| ACTB || 1:1000 || treated || 32.862 || --- || --- | |||
|- | |||
| ACTB || 1:1000 || untreated || 34.823 || --- || --- | |||
|} | |} | ||
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__NOTOC__ | __NOTOC__ |
Revision as of 12:52, 11 July 2014
Cell Fate Switch by Synthetic Transcription Factors | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RT-PCR E004Target Genes
Results
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