User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/06/25: Difference between revisions

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* [http://openwetware.org/wiki/Haynes:SYBRGreen Standard Procedure: SYBRGreen Assay]
* [http://openwetware.org/wiki/Haynes:SYBRGreen Standard Procedure: SYBRGreen Assay]


===Target Genes===
{| {{table}}  
{| {{table}}  
|- bgcolor=#cfcfcf  
|- bgcolor=#cfcfcf  
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|-
|-
| nTc || --- || A,B,C,D,E,F,G,H8-10 || ---
| nTc || --- || A,B,C,D,E,F,G,H8-10 || ---
|}
===Results===
{| {{table}}
|- bgcolor=#cfcfcf
| Gene || dilution || +/- PcTF || Mean CP || delta CP || double delta CP
|-
| PDX1  || 1:10 || treated || 0 || 0 || Invalid
|-
| PDX1  || 1:10 || untreated || 0 || 0 || 0
|-
| MAFA || 1:10 || treated || 34.440 || 0.3350 || 0.3624
|-
| MAFA || 1:10 || untreated || 34.936 || 0.9245 || 1
|-
| GLP1R  || 1:10 || treated || 40 || 7.10E-3 || 0.2568
|-
| GLP1R  || 1:10 || untreated || 40 || 2.76E-2 || 1
|-
| PCSK1  || 1:10 || treated || 0 || Invalid || Invalid
|-
| PCSK1  || 1:10 || untreated || 0 || Invalid || Invalid
|-
| IAPP  || 1:10 || treated || 38.363 || 2.21E-2 || 3.14E-2
|-
| IAPP  || 1:10 || untreated || 35.333 || 0.7022 || 1
|-
| KCNQ2  || 1:10 || treated || 31.621 || 2.363 || 0.5395
|-
| KCNQ2  || 1:10 || untreated || 32.6919 || 4.381 || 1
|-
| mCh  || 1:1000 || treated || 33.3063 || 0.7350 || Invalid
|-
| mCh  || 1:1000 || untreated || 0 || 0 || Invalid
|-
| ACTB || 1:1000 || treated || 32.862 || --- || ---
|-
| ACTB || 1:1000 || untreated || 34.823 || --- || ---
|}
|}
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__NOTOC__
__NOTOC__

Revision as of 12:52, 11 July 2014

Cell Fate Switch by Synthetic Transcription Factors <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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RT-PCR E004

Target Genes

Gene dilution RTPCR number +/- PcTF
PDX1 1:10 A1-3 treated
PDX1 1:10 A4-7 untreated
MAFA 1:10 B1-3 treated
MAFA 1:10 B4-7 untreated
GLP1R 1:10 C1-3 treated
GLP1R 1:10 C4-7 untreated
PCSK1 1:10 D1-3 treated
PCSK1 1:10 D4-7 untreated
IAPP 1:10 E1-3 treated
IAPP 1:10 E4-7 untreated
KCNQ2 1:10 F1-3 treated
KCNQ2 1:10 F4-7 untreated
mCh 1:1000 G1-3 treated
mCh 1:1000 G4-7 untreated
ACTB 1:1000 H1-3 treated
ACTB 1:1000 H4-7 untreated
nTc --- A,B,C,D,E,F,G,H8-10 ---

Results

Gene dilution +/- PcTF Mean CP delta CP double delta CP
PDX1 1:10 treated 0 0 Invalid
PDX1 1:10 untreated 0 0 0
MAFA 1:10 treated 34.440 0.3350 0.3624
MAFA 1:10 untreated 34.936 0.9245 1
GLP1R 1:10 treated 40 7.10E-3 0.2568
GLP1R 1:10 untreated 40 2.76E-2 1
PCSK1 1:10 treated 0 Invalid Invalid
PCSK1 1:10 untreated 0 Invalid Invalid
IAPP 1:10 treated 38.363 2.21E-2 3.14E-2
IAPP 1:10 untreated 35.333 0.7022 1
KCNQ2 1:10 treated 31.621 2.363 0.5395
KCNQ2 1:10 untreated 32.6919 4.381 1
mCh 1:1000 treated 33.3063 0.7350 Invalid
mCh 1:1000 untreated 0 0 Invalid
ACTB 1:1000 treated 32.862 --- ---
ACTB 1:1000 untreated 34.823 --- ---


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