User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/09/17: Difference between revisions

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(Autocreate 2014/09/17 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors)
 
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==Entry title==
==Transfer Thawed (0), TC1Alpha(2)==
* Insert content here...
* 65% confluent T-25 flask. Cells passaged from thawed (9/5).  
* Transfer cells into one well in a 6 well plate.
* Used Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells]
* Volumes changed: 2 mL PBS, 0.5 mL Trypsin, 3.5 mL DMEM.
* 4 mL transferred into 1 well.
* Cells to be grown in 10cm dishes after confluence with well.




Revision as of 11:23, 17 September 2014

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Transfer Thawed (0), TC1Alpha(2)

  • 65% confluent T-25 flask. Cells passaged from thawed (9/5).
  • Transfer cells into one well in a 6 well plate.
  • Used Protocol: Splitting Cells
  • Volumes changed: 2 mL PBS, 0.5 mL Trypsin, 3.5 mL DMEM.
  • 4 mL transferred into 1 well.
  • Cells to be grown in 10cm dishes after confluence with well.



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