User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/13: Difference between revisions

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(Autocreate 2014/11/13 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors)
 
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==Entry title==
==Pancreas Cell Culture==
* Insert content here...
* Day 8. Changed cell media in t-25.  
# Aspirate off current media
# Add 4mL (37 C) 10% FBS DMEM
 
*Should look into increasing FBS concentration to create better growth environment.  
*Quantify needed confluence to effectively split cells (maybe 1:5 or 1:4)




Revision as of 10:16, 14 November 2014

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Pancreas Cell Culture

  • Day 8. Changed cell media in t-25.
  1. Aspirate off current media
  2. Add 4mL (37 C) 10% FBS DMEM
  • Should look into increasing FBS concentration to create better growth environment.
  • Quantify needed confluence to effectively split cells (maybe 1:5 or 1:4)



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