User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/17: Difference between revisions
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(Autocreate 2014/11/17 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors) |
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== | ==ELISA (test)== | ||
* | * Continuing Protocol from NAP blocking stage. Protocol [http://openwetware.org/wiki/Haynes:ELISA here] | ||
* Antibodies: | |||
*FL180 (Santa Cruz Biotech)- Glucagon (concentration 200ug/mL) - diluted 1/50 | |||
*ab7842 (ABCAM) - Insulin (concentration 200ug/mL) - diluted 1/50 | |||
*Insulin primary not meant for ELISA? Testing anyways | |||
==Pancreatic Cell Culture== | |||
* Cells look near 60% confluence - FBS worked? | |||
==RT-PCR== | |||
* Dilutions of Target Primers to 1/10 (100uM) | |||
Revision as of 12:38, 17 November 2014
 Cell Fate Switch by Synthetic Transcription Factors
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ELISA (test)
*FL180 (Santa Cruz Biotech)- Glucagon (concentration 200ug/mL) - diluted 1/50 *ab7842 (ABCAM) - Insulin (concentration 200ug/mL) - diluted 1/50
Pancreatic Cell Culture
RT-PCR
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