User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/18

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Cell Fate Switch by Synthetic Transcription Factors <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Pancreatic Cell Culture

  • Insert content here...

RT-PCR

  • Protocol
  • Note on bench to make 750nM primers

ELISA

Data Analysis

  • Experiment showed no concentration of Insulin or Glucagon (low absorbance)
  • Control experiment (only substrate) turned very blue (high absorbance)

           Added TBST in last washing step - activation of substrate?



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