User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/18: Difference between revisions
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==ELISA== | ==ELISA== | ||
===Data Analysis=== | ====Data Analysis===== | ||
* Experiment showed no concentration of Insulin or Glucagon (low absorbance) | * Experiment showed no concentration of Insulin or Glucagon (low absorbance) | ||
* Control experiment (only substrate) turned very blue (high absorbance) | * Control experiment (only substrate) turned very blue (high absorbance) | ||
Added TBST in last washing step - activation of substrate? | Added TBST in last washing step - activation of substrate? | ||
==Bioinformatics== | |||
====GO Term Analysis==== | |||
* Should go over GO Terms again | |||
* G-protein linked to secretion, acceptance of glucagon/insulin (importance) | |||
* What GO Terms should we see? | |||
GSIS, Secretion, Packaging, Membrance Permeation Proteins... | |||
Revision as of 15:23, 18 November 2014
 Cell Fate Switch by Synthetic Transcription Factors
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Pancreatic Cell Culture
RT-PCR
ELISAData Analysis=
Added TBST in last washing step - activation of substrate? BioinformaticsGO Term Analysis
GSIS, Secretion, Packaging, Membrance Permeation Proteins...
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