User:Derek Thirstrup: Difference between revisions

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[[Image:OWWEmblem.png|thumb|right|Derek Thirstrup (an artistic interpretation)]]
[[Image:OWWEmblem.png|thumb|right|Derek Thirstrup (an artistic interpretation)]]


*Derek Thirstrup, Research Scientist IV
*Derek Thirstrup, Scientist
*Lab of Dr. Geoffrey Baird
*Presage Biosciences
*Department of Laboratory Medicine
*Ninth & Jefferson Building, Harborview Medical Center
*University of Washington, Seattle, WA
*[[Special:Emailuser/Derek Thirstrup|Email me through OpenWetWare]]


I am a Research Scientist in Geoffrey Baird's molecular pathology research lab at the University of Washington. 


==Education==
==Education==
Line 19: Line 14:
<!-- Feel free to add brief descriptions to your research interests as well -->
<!-- Feel free to add brief descriptions to your research interests as well -->


# Co-inventor of key enabling techniques used in rapid Aptamer HistoChemistry (AHC) diagnostic pathology tissue staining method
# Co-inventor of key enabling techniques used for rapid Aptamer HistoChemistry (AHC) diagnostic pathology applications
# Biomarker validation utilizing machine learning based fluorescent image scoring methods
# Biomarker validation utilizing custom supervised machine learning based tissue image classifiers
# Luminex assay development
# siRNA cell transfection technology
# siRNA cell transfection technology
# 3D spheroid tissue culture


==Publications==
==Publications==
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->


1.  Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition.
1.  MyoD is a tumor suppressor gene in Medulloblastoma.
    Joyoti Dey, Adrian M. Dubuc, Kyle D Pedro, Derek Thirstrup, Brigham H Mecham, Paul Northcott, Xiaochong Wu, David J. Shih, Stephen J. Tapscott, Michael L. LeBlanc, Michael D. Taylor, and James M. Olson.
    Cancer Res. doi: 10.1158/0008-5472.CAN-13-0730-T. Published online October 3, 2013.
    http://cancerres.aacrjournals.org/content/early/2013/10/03/0008-5472.CAN-13-0730-T.abstract
 
2.  Independent, Parallel Pathways to CXCL10 Induction in HCV-Infected Hepatocytes.
    Brownell J, Wagoner J, Lovelace ES, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Nicholas CI, Rosen HR, Polyak SJ.
    J Hepatol. 58(6): doi:10.1016/j.jhep.2013.06.001. Published online June 13, 2013.
    http://www.journal-of-hepatology.eu/article/S0168-8278(13)00378-4/abstract
 
3.  Protein Signature of Lung Cancer Tissues.
    Mehan MR, Ayers D, Thirstrup D, Xiong W, Ostroff RM, Brody EN, Walker JJ, Gold L, Jarvis TC, Janjic N, Baird GS, Wilcox SK.
    PLOS One 7(4): e35157. doi:10.1371/journal.pone.0035157. Published online Apr 11, 2012.
    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035157
 
4.  Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition.
     Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J, Zhang C, Gelinas A, Weiss A, Janjic N, Baird GS.
     Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J, Zhang C, Gelinas A, Weiss A, Janjic N, Baird GS.
     Appl Immunohistochem Mol Morphol. 2011 Jan 7. [Epub ahead of print]
     Appl Immunohistochem Mol Morphol. 2011 Jan 7. [Epub ahead of print]
    http://journals.lww.com/appliedimmunohist/pages/articleviewer.aspx?year=2011&issue=05000&article=00012&type=abstract


2.  Efficacy of TRAIL treatment against HPV16 infected cervical cancer cells undergoing senescence following siRNA knockdown of E6/E7 genes.
5.  Efficacy of TRAIL treatment against HPV16 infected cervical cancer cells undergoing senescence following siRNA knockdown of E6/E7 genes.
     Eaton S, Wiktor P, Thirstrup D, Lake D, Nagaraj VJ.
     Eaton S, Wiktor P, Thirstrup D, Lake D, Nagaraj VJ.
     Biochem Biophys Res Commun. 2010 Dec 16. [Epub ahead of print]
     Biochem Biophys Res Commun. 2010 Dec 16. [Epub ahead of print]
    http://www.sciencedirect.com/science/article/pii/S0006291X10022941


3.  Histochemical application of a peroxidase DNAzyme with a covalently attached hemin cofactor.
6.  Histochemical application of a peroxidase DNAzyme with a covalently attached hemin cofactor.
     Thirstrup D, Baird GS.
     Thirstrup D, Baird GS.
     Anal Chem. 2010 Mar 15;82(6):2498-504.
     Anal Chem. 2010 Mar 15;82(6):2498-504.
    http://pubs.acs.org/doi/abs/10.1021/ac902887j


4.  Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis.
7.  Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis.
     Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P.
     Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P.
     Biochem Biophys Res Commun. 2008 Oct 31;375(4):526-30. Epub 2008 Aug 19.
     Biochem Biophys Res Commun. 2008 Oct 31;375(4):526-30. Epub 2008 Aug 19.
    http://www.sciencedirect.com/science/article/pii/S0006291X08015672
==Meetings==
6th Principal Investigators Meeting
Innovative Molecular Analysis Technologies Program
National Cancer Institute
September 7-9, 2005
http://imat.cancer.gov/news/b.asp
7th Principal Investigators Meeting
Innovative Molecular Analysis Technologies Program
National Cancer Institute
September 7-8, 2006
http://imat.cancer.gov/news/7th_roster.asp
==Aptamer stained tissue section images==
HER2 aptamer stained breast cancer 5um tissue section image http://picasaweb.google.com/derek.thirstrup/DropBox?authkey=Gv1sRgCNbfwtuNvq-quwE&pli=1&gsessionid=DR3nNqBzfPpxnZiAZ-AsnQ#5568785165955274322
EGFR aptamer stained skin 5um tissue section image https://picasaweb.google.com/lh/photo/Hg3IUdPB9h_8T8NoQqZudSHj9xFgnYa4zrNk-WJtUVI?feat=directlink


==Useful links==
==Useful links==
HER2 aptamer tissue section image http://picasaweb.google.com/derek.thirstrup/DropBox?authkey=Gv1sRgCNbfwtuNvq-quwE&pli=1&gsessionid=DR3nNqBzfPpxnZiAZ-AsnQ#5568785165955274322
http://www.proteinatlas.org/
 
*http://www.proteinatlas.org/

Latest revision as of 20:08, 6 October 2013

Contact Info

Derek Thirstrup (an artistic interpretation)
  • Derek Thirstrup, Scientist
  • Presage Biosciences


Education

  • 2002, MS, University of Washington, Analytical Chemistry
  • 2000, BS, South Dakota School of Mines & Technology, Chemistry

Research interests

  1. Co-inventor of key enabling techniques used for rapid Aptamer HistoChemistry (AHC) diagnostic pathology applications
  2. Biomarker validation utilizing custom supervised machine learning based tissue image classifiers
  3. Luminex assay development
  4. siRNA cell transfection technology
  5. 3D spheroid tissue culture

Publications

1. MyoD is a tumor suppressor gene in Medulloblastoma. 

    Joyoti Dey, Adrian M. Dubuc, Kyle D Pedro, Derek Thirstrup, Brigham H Mecham, Paul Northcott, Xiaochong Wu, David J. Shih, Stephen J. Tapscott, Michael L. LeBlanc, Michael D. Taylor, and James M. Olson. 
    Cancer Res. doi: 10.1158/0008-5472.CAN-13-0730-T. Published online October 3, 2013.
    http://cancerres.aacrjournals.org/content/early/2013/10/03/0008-5472.CAN-13-0730-T.abstract

2. Independent, Parallel Pathways to CXCL10 Induction in HCV-Infected Hepatocytes. 

    Brownell J, Wagoner J, Lovelace ES, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Nicholas CI, Rosen HR, Polyak SJ. 
    J Hepatol. 58(6): doi:10.1016/j.jhep.2013.06.001. Published online June 13, 2013.
    http://www.journal-of-hepatology.eu/article/S0168-8278(13)00378-4/abstract

3. Protein Signature of Lung Cancer Tissues. 

    Mehan MR, Ayers D, Thirstrup D, Xiong W, Ostroff RM, Brody EN, Walker JJ, Gold L, Jarvis TC, Janjic N, Baird GS, Wilcox SK. 
    PLOS One 7(4): e35157. doi:10.1371/journal.pone.0035157. Published online Apr 11, 2012.
    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035157

4. Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition. 

    Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J, Zhang C, Gelinas A, Weiss A, Janjic N, Baird GS.
    Appl Immunohistochem Mol Morphol. 2011 Jan 7. [Epub ahead of print]
    http://journals.lww.com/appliedimmunohist/pages/articleviewer.aspx?year=2011&issue=05000&article=00012&type=abstract

5. Efficacy of TRAIL treatment against HPV16 infected cervical cancer cells undergoing senescence following siRNA knockdown of E6/E7 genes. 

    Eaton S, Wiktor P, Thirstrup D, Lake D, Nagaraj VJ.
    Biochem Biophys Res Commun. 2010 Dec 16. [Epub ahead of print]
    http://www.sciencedirect.com/science/article/pii/S0006291X10022941

6. Histochemical application of a peroxidase DNAzyme with a covalently attached hemin cofactor. 

    Thirstrup D, Baird GS.
    Anal Chem. 2010 Mar 15;82(6):2498-504.
    http://pubs.acs.org/doi/abs/10.1021/ac902887j

7. Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis. 

    Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P.
    Biochem Biophys Res Commun. 2008 Oct 31;375(4):526-30. Epub 2008 Aug 19.
    http://www.sciencedirect.com/science/article/pii/S0006291X08015672

Meetings

6th Principal Investigators Meeting

Innovative Molecular Analysis Technologies Program

National Cancer Institute

September 7-9, 2005

http://imat.cancer.gov/news/b.asp


7th Principal Investigators Meeting

Innovative Molecular Analysis Technologies Program

National Cancer Institute

September 7-8, 2006

http://imat.cancer.gov/news/7th_roster.asp

Aptamer stained tissue section images

HER2 aptamer stained breast cancer 5um tissue section image http://picasaweb.google.com/derek.thirstrup/DropBox?authkey=Gv1sRgCNbfwtuNvq-quwE&pli=1&gsessionid=DR3nNqBzfPpxnZiAZ-AsnQ#5568785165955274322

EGFR aptamer stained skin 5um tissue section image https://picasaweb.google.com/lh/photo/Hg3IUdPB9h_8T8NoQqZudSHj9xFgnYa4zrNk-WJtUVI?feat=directlink

Useful links

http://www.proteinatlas.org/

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