User:Derek Thirstrup
Contact Info
- Derek Thirstrup, Research Scientist IV
- Lab of Dr. Geoffrey Baird
- Department of Laboratory Medicine
- Ninth & Jefferson Building, Harborview Medical Center
- University of Washington, Seattle, WA
- Email me through OpenWetWare
Education
- 2002, MS, University of Washington, Analytical Chemistry
- 2000, BS, South Dakota School of Mines & Technology, Chemistry
Research interests
- Co-inventor of key enabling techniques used for rapid Aptamer HistoChemistry (AHC) diagnostic pathology applications
- Biomarker validation utilizing custom supervised machine learning based tissue image classifiers
- Luminex assay development
- siRNA cell transfection technology
- 3D spheroid tissue culture
Publications
1. Protein Signature of Lung Cancer Tissues.
Mehan MR, Ayers D, Thirstrup D, Xiong W, Ostroff RM, Brody EN, Walker JJ, Gold L, Jarvis TC, Janjic N, Baird GS, Wilcox SK.
PLOS One 7(4): e35157. doi:10.1371/journal.pone.0035157. Published online Apr 11, 2012.
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035157
2. Rapid Histochemistry Using Slow Off-rate Modified Aptamers With Anionic Competition.
Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J, Zhang C, Gelinas A, Weiss A, Janjic N, Baird GS.
Appl Immunohistochem Mol Morphol. 2011 Jan 7. [Epub ahead of print]
3. Efficacy of TRAIL treatment against HPV16 infected cervical cancer cells undergoing senescence following siRNA knockdown of E6/E7 genes.
Eaton S, Wiktor P, Thirstrup D, Lake D, Nagaraj VJ.
Biochem Biophys Res Commun. 2010 Dec 16. [Epub ahead of print]
4. Histochemical application of a peroxidase DNAzyme with a covalently attached hemin cofactor.
Thirstrup D, Baird GS.
Anal Chem. 2010 Mar 15;82(6):2498-504.
5. Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis.
Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P.
Biochem Biophys Res Commun. 2008 Oct 31;375(4):526-30. Epub 2008 Aug 19.
Meetings
6th Principal Investigators Meeting
Innovative Molecular Analysis Technologies Program
National Cancer Institute
September 7-9, 2005
http://imat.cancer.gov/news/b.asp
7th Principal Investigators Meeting
Innovative Molecular Analysis Technologies Program
National Cancer Institute
September 7-8, 2006
http://imat.cancer.gov/news/7th_roster.asp
Aptamer stained tissue section images
HER2 aptamer stained breast cancer 5um tissue section image http://picasaweb.google.com/derek.thirstrup/DropBox?authkey=Gv1sRgCNbfwtuNvq-quwE&pli=1&gsessionid=DR3nNqBzfPpxnZiAZ-AsnQ#5568785165955274322
EGFR aptamer stained skin 5um tissue section image https://picasaweb.google.com/lh/photo/Hg3IUdPB9h_8T8NoQqZudSHj9xFgnYa4zrNk-WJtUVI?feat=directlink
