User:Desiree Carmen/Notebook/AU Biomaterial Lab/2014/04/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
 
* Insert content here...
 
 
 
==Objective==
Synthesize Mn-doped ZnS quantum dots using the synthesis described [http://pubs.acs.org/doi/full/10.1021/nn2024896 here]
 
==Description==
Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM
 
# Add 176μL of 5mM Zn(CH<sub>3</sub>COO)<sub>2</sub> to a test tube
# Add 24μL of 5mM Mn((CH<sub>3</sub>COO)<sub>2</sub>) to the test tube
# Add 200μL of 40mM NH4CH<sub>3</sub>COO to the test tube
# Add 244μL of 0.55mg/mL protein to the test tube
# Add 250μL of 10mM NH<sub>4</sub>OH
## This should bring the pH up to 8.2. Check on this with a pH strip (290mM 10mM NH<sub>4</sub>OH)
# Bring the volume up to 2.5mL with water (Add 1316μL of water)
# Incubate at room temperature for 1 hour
# Add 200μL of 5mM Na<sub>2</sub>S
## When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
# Incubate at 37C for 5 days.
 
==Data==
<u>Stock Solutions</u>
 
All solutions are made in 250mL volumetric flasks.
 
#5mM Zn(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 219.49 g/mol
## V = 250mL
## mass to be measured = 0.274g
## actual mass = 0.274g
## actual concentration = 5mM
#5mM Mn(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 173.02 g/mol
## V = 250mL
## mass to be measured = 0.216g
## actual mass = 0.220 g (slightly pinkish hue)
## actual concentration = 5.1mM
#40mM NH<sub>4</sub>(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 77.08 g/mol
## V = 250mL
## mass to be measured = 0.7708g
## actual mass = 0.780g
## actual concentration = 40.5mM
#10mM NH<sub>4</sub>OH
## initial M = 14.5M
## final V = 250mL
## volume to be diluted = 172μL
## actual concentration =
#5mM Na<sub>2</sub>S
## FW = 240.18 g/mol
## V = 250mL
## mass to be measured = 0.300g
## actual mass = 0.300g
## actual concentration = 5.00mM
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 




Revision as of 09:03, 11 April 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>



Objective

Synthesize Mn-doped ZnS quantum dots using the synthesis described here

Description

Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM

  1. Add 176μL of 5mM Zn(CH3COO)2 to a test tube
  2. Add 24μL of 5mM Mn((CH3COO)2) to the test tube
  3. Add 200μL of 40mM NH4CH3COO to the test tube
  4. Add 244μL of 0.55mg/mL protein to the test tube
  5. Add 250μL of 10mM NH4OH
    1. This should bring the pH up to 8.2. Check on this with a pH strip (290mM 10mM NH4OH)
  6. Bring the volume up to 2.5mL with water (Add 1316μL of water)
  7. Incubate at room temperature for 1 hour
  8. Add 200μL of 5mM Na2S
    1. When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
  9. Incubate at 37C for 5 days.

Data

Stock Solutions

All solutions are made in 250mL volumetric flasks.

  1. 5mM Zn(CH3COO)2
    1. FW = 219.49 g/mol
    2. V = 250mL
    3. mass to be measured = 0.274g
    4. actual mass = 0.274g
    5. actual concentration = 5mM
  2. 5mM Mn(CH3COO)2
    1. FW = 173.02 g/mol
    2. V = 250mL
    3. mass to be measured = 0.216g
    4. actual mass = 0.220 g (slightly pinkish hue)
    5. actual concentration = 5.1mM
  3. 40mM NH4(CH3COO)2
    1. FW = 77.08 g/mol
    2. V = 250mL
    3. mass to be measured = 0.7708g
    4. actual mass = 0.780g
    5. actual concentration = 40.5mM
  4. 10mM NH4OH
    1. initial M = 14.5M
    2. final V = 250mL
    3. volume to be diluted = 172μL
    4. actual concentration =
  5. 5mM Na2S
    1. FW = 240.18 g/mol
    2. V = 250mL
    3. mass to be measured = 0.300g
    4. actual mass = 0.300g
    5. actual concentration = 5.00mM

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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