User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
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*A spectrum of 1mM adenosine was taken. The absorbance exceeded the capabilities of the spectrometer, so 100μM adenosine (0.6mL 500μM stock adenosine diluted with 100μM by adding 2.4mL buffer) was used as the maximum concentration. | *A spectrum of 1mM adenosine was taken. The absorbance exceeded the capabilities of the spectrometer, so 100μM adenosine (0.6mL 500μM stock adenosine diluted with 100μM by adding 2.4mL buffer) was used as the maximum concentration. | ||
*Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | *Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | ||
**The velocity was | **The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity). | ||
==Observations== | ==Observations== |
Revision as of 12:14, 5 February 2013
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Objective
Calculations
Procedure
Observations |