User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23

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(Objective)
Current revision (15:48, 8 May 2013) (view source)
(Experimental Protocol)
 
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==Experimental Protocol==
 
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'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
 
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*Add 3.1 g of NaH<sub>2</sub>PO<sub>4</sub>•H<sub>2</sub>O and 10.9 g of Na<sub>2</sub>HPO<sub>4</sub> (anhydrous) to distilled H<sub>2</sub>O to make a volume of 1 L.
 
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**The pH of the final solution will be 7.4.
 
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**This buffer can be stored for up to 1 mo at 4°C.
 
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*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H<sub>2</sub>O)
 
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'''Running ADA Kinetics'''
 
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*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
 
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[[Image:ADA_kinetics_table.png|px80]]
 
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*To prepare a 30 mM stock solution of adenosine:
 
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<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
 
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*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
 
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