User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/29 - Revision history

Mary Mendoza: /* Description */

Mary Mendoza — Wed, 08 May 2013 20:51:30 GMT

Description

←Older revision		Revision as of 20:51, 8 May 2013
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-	~~==Description==~~
-	~~1. Reallocating ADA~~
-	*Measure 2.5mg was added to a tube and stored at 4°C
-	**0.5U/(19.8U/mg) = 0.02525mg
-
-	~~2. 0.05M Sodium Phosphate Buffer (pH 7.4)~~
-	*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L, 1M Sodium Phosphate Buffer.
-	**The pH of the solution was 7.03, so 1M NaOH was added drop-wise until the solution reached a pH of 7.4.
-	**This buffer was stored at 4°C.
-	*50mL of the 1M Sodium Phosphate Buffer was diluted in 1L H<sub>2</sub>O.
-
-	~~3. Preparing a stock solution of adenosine~~
-	*Initially, 0.0802g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 30mM solution), however the solution was super saturated.
-	*Then, 0.0401g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 15mM solution), but the solution was still saturated.
-	**5mL of 0.05M Sodium Phosphate Buffer was added to this solution in order (to make 10mM solution), but one flake of adenosine remained out of solution.
-	**5mL of 0.05M Sodium Phosphate Buffer was added to the 10mM solution (to make 5mM solution) and the adenosine was fully dissolved in solution.
-	*The final concentration of the adenosine stock solution was 5mM.

Mary Mendoza: /* Objective */

Mary Mendoza — Wed, 08 May 2013 20:51:14 GMT

Objective

←Older revision		Revision as of 20:51, 8 May 2013
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-	~~==Objective==~~
-	*to reallocate ADA into 0.0253mg per tube
-	*to prepare phosphate buffer
-	*to prepare adenosine stock solution
-	*to prepare ADA stock solution for today
-	*to run ADA kinetics using UV-vis

Dhea Patel at 19:13, 13 February 2013

Dhea Patel — Wed, 13 Feb 2013 19:13:04 GMT

←Older revision		Revision as of 19:13, 13 February 2013
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		+	\|-
		+	\|colspan="2" style="background-color: #F2F2F2;" align="right"\|[[{{FULLPAGENAME}}/Entry_Base\|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page\|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
		+
	==Objective==		==Objective==
	*to reallocate ADA into 0.0253mg per tube		*to reallocate ADA into 0.0253mg per tube

Dhea Patel: /* Description */

Dhea Patel — Wed, 30 Jan 2013 16:52:48 GMT

Description

←Older revision		Revision as of 16:52, 30 January 2013
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	==Description==		==Description==
	1. Reallocating ADA		1. Reallocating ADA
-	*~~measure 0~~.~~0253mg into each~~ tube.	+	*Measure 2.5mg was added to a tube and stored at 4°C
	**0.5U/(19.8U/mg) = 0.02525mg		**0.5U/(19.8U/mg) = 0.02525mg
		+
		+	2. 0.05M Sodium Phosphate Buffer (pH 7.4)
		+	*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L, 1M Sodium Phosphate Buffer.
		+	**The pH of the solution was 7.03, so 1M NaOH was added drop-wise until the solution reached a pH of 7.4.
		+	**This buffer was stored at 4°C.
		+	*50mL of the 1M Sodium Phosphate Buffer was diluted in 1L H<sub>2</sub>O.
		+
		+	3. Preparing a stock solution of adenosine
		+	*Initially, 0.0802g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 30mM solution), however the solution was super saturated.
		+	*Then, 0.0401g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 15mM solution), but the solution was still saturated.
		+	**5mL of 0.05M Sodium Phosphate Buffer was added to this solution in order (to make 10mM solution), but one flake of adenosine remained out of solution.
		+	**5mL of 0.05M Sodium Phosphate Buffer was added to the 10mM solution (to make 5mM solution) and the adenosine was fully dissolved in solution.
		+	*The final concentration of the adenosine stock solution was 5mM.
		+

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: /* Objective */

Dhea Patel — Tue, 29 Jan 2013 18:13:15 GMT

Objective

←Older revision		Revision as of 18:13, 29 January 2013
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	*to run ADA kinetics using UV-vis		*to run ADA kinetics using UV-vis

		+
		+	==Description==
		+	1. Reallocating ADA
		+	*measure 0.0253mg into each tube.
		+	**0.5U/(19.8U/mg) = 0.02525mg

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: /* Objective */

Dhea Patel — Tue, 29 Jan 2013 17:16:22 GMT

Objective

←Older revision		Revision as of 17:16, 29 January 2013
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	==Objective==		==Objective==
-	*to reallocate ADA into ~~[[]]~~ per tube	+	*to reallocate ADA into 0.0253mg per tube
	*to prepare phosphate buffer		*to prepare phosphate buffer
	*to prepare adenosine stock solution		*to prepare adenosine stock solution

Dhea Patel at 17:11, 29 January 2013

Dhea Patel — Tue, 29 Jan 2013 17:11:12 GMT

←Older revision		Revision as of 17:11, 29 January 2013
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Objective==
-	* ~~Insert content here...~~	+	*to reallocate ADA into [[]] per tube
		+	*to prepare phosphate buffer
		+	*to prepare adenosine stock solution
		+	*to prepare ADA stock solution for today
		+	*to run ADA kinetics using UV-vis

Dhea Patel: Autocreate 2013/01/29 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics

Dhea Patel — Tue, 29 Jan 2013 17:09:33 GMT

Autocreate 2013/01/29 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__