User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05

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==Objective==
 
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*to perform ADA kinetics assays using UV-vis.
 
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==Calculations==
 
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*ADA Kinetics were run using the following volumes and concentrations.
 
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**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.
 
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[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]]
 
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*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.
 
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==Procedure==
 
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#UVProbe was opened.
 
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##Window > 1. Kinetics
 
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##Methods Icon
 
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###Wavelength: 265nm
 
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###Duration: 600 seconds (5minutes)
 
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###OK
 
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#Shimadzu CPS-Controller was set to 25°C.
 
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##wait for the temperature to raise to 25°C
 
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#place the sample in the cell and click start.
 
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==Observations==
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