User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05

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(Procedure)
Current revision (15:55, 8 May 2013) (view source)
(Data)
 
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==Data==
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Average Velocity over 30s (nm/sec)'''
 
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|-
 
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| 100||0.000345161
 
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|-
 
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| 10||0.000109677
 
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|-
 
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| 1||2.58065E-05
 
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|}
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''1/[adenosine] (1/uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/[velocity] (sec/nm)'''
 
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|-
 
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| 0.01||2897.196262
 
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|-
 
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| 0.1||9117.647059
 
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|-
 
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| 1||38750
 
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|}
 
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Lineweaver-Burk Plot of the data obtained today.
 
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[[Image:Lineweaver-Burk_Plot_2013-02-05.png]]
 
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{| {{table}}
 
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| 1/Vmax||4015.59923
 
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|-
 
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| '''Vmax'''||'''0.000249029 nm/sec'''
 
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|-
 
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| 1/Km||0.111964094
 
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|-
 
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| '''Km'''||'''8.93143474 uM'''
 
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|}
 
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