User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
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##wait for the temperature to raise to 25°C | ##wait for the temperature to raise to 25°C | ||
#place the sample in the cell and click start. | #place the sample in the cell and click start. | ||
*Phosphate buffer was used as a blank and was used to create the baseline. | |||
*A spectrum of 1mM adenosine was taken. The absorbance exceeded the capabilities of the spectrometer, so 100μM adenosine (0.6mL 500μM stock adenosine diluted with 100μM by adding 2.4mL buffer) was used as the maximum concentration. | |||
*Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | |||
**The velocity was calculated to be | |||
==Observations== | ==Observations== |
Revision as of 12:05, 5 February 2013
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Objective
Calculations
Procedure
Observations |