User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05

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(Autocreate 2013/02/05 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics)
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==Entry title==
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==Objective==
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* Insert content here...
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*to perform ADA kinetics assays using UV-vis.  
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==Calculations==
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*ADA Kinetics were run using the following volumes and concentrations.
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**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.
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[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]]
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*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.
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==Procedure==
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#UVProbe was opened.
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##Window > 1. Kinetics
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##Methods Icon
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###Wavelength: 265nm
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###Duration: 600 seconds (5minutes)
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###OK
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#Shimadzu CPS-Controller was set to 25°C.
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##wait for the temperature to raise to 25°C
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#place the sample in the cell and click start.
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==Observations==
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Revision as of 13:46, 5 February 2013

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Objective

  • to perform ADA kinetics assays using UV-vis.

Calculations

  • ADA Kinetics were run using the following volumes and concentrations.
    • Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.

Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png

  • The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.

Procedure

  1. UVProbe was opened.
    1. Window > 1. Kinetics
    2. Methods Icon
      1. Wavelength: 265nm
      2. Duration: 600 seconds (5minutes)
      3. OK
  2. Shimadzu CPS-Controller was set to 25°C.
    1. wait for the temperature to raise to 25°C
  3. place the sample in the cell and click start.

Observations


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