User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions

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(Autocreate 2013/02/05 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics)
 
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==Entry title==
==Objective==
* Insert content here...
*to perform ADA kinetics assays using UV-vis.  


==Calculations==
*ADA Kinetics were run using the following volumes and concentrations.
**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.
[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]]
*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.
==Procedure==
#UVProbe was opened.
##Window > 1. Kinetics
##Methods Icon
###Wavelength: 265nm
###Duration: 600 seconds (5minutes)
###OK
#Shimadzu CPS-Controller was set to 25°C.
##wait for the temperature to raise to 25°C
#place the sample in the cell and click start.
==Observations==


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Revision as of 10:46, 5 February 2013

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Objective

  • to perform ADA kinetics assays using UV-vis.

Calculations

  • ADA Kinetics were run using the following volumes and concentrations.
    • Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.

  • The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.

Procedure

  1. UVProbe was opened.
    1. Window > 1. Kinetics
    2. Methods Icon
      1. Wavelength: 265nm
      2. Duration: 600 seconds (5minutes)
      3. OK
  2. Shimadzu CPS-Controller was set to 25°C.
    1. wait for the temperature to raise to 25°C
  3. place the sample in the cell and click start.

Observations


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