User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
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**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette. | **This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette. | ||
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]] | ***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]] | ||
==Data== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 30s (nm/sec)''' | |||
|- | |||
| 100||0.000345161 | |||
|- | |||
| 10||0.000109677 | |||
|- | |||
| 1||2.58065E-05 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/[adenosine] (1/uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/[velocity] (sec/nm)''' | |||
|- | |||
| 0.01||2897.196262 | |||
|- | |||
| 0.1||9117.647059 | |||
|- | |||
| 1||38750 | |||
|} | |||
Lineweaver-Burk Plot of the data obtained today. | |||
[[Image:Lineweaver-Burk_Plot_2013-02-05.png]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/Vmax''' | |||
| align="center" style="background:#f0f0f0;"|'''4015.59923''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Vmax||0.000249029||nm/sec | |||
|- | |||
| 1/Km||0.111964094|| | |||
|- | |||
| Km||8.93143474||uM | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 19:27, 5 February 2013
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Objective
Calculations
Procedure
Data
Lineweaver-Burk Plot of the data obtained today.
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