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Project name
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Objective
- to perform ADA kinetics assays using UV-vis.
Calculations
- ADA Kinetics were run using the following volumes and concentrations.
- Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.
- The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.
Procedure
- UVProbe was opened.
- Window > 1. Kinetics
- Methods Icon
- Wavelength: 265nm
- Duration: 600 seconds (5minutes)
- OK
- Shimadzu CPS-Controller was set to 25°C.
- wait for the temperature to raise to 25°C
- place the sample in the cell and click start.
Observations
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