User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

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< User:Dhea Patel | Notebook | CHEM 572: ADA&Inhibitor Kinetics | 2013 | 02(Difference between revisions)
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==Objective==
 
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*to finish Trail 2 and run Trial 3 of ADA kinetics
 
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==Description==
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*to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
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**keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
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*The following table was used to create the mixtures in the cuvette.
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**[[Image:Data_Table.JPG]]
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**In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
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**All the listed concentrations were run for trial 3.
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