User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13
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*to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm. | *to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm. | ||
**keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell. | **keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell. | ||
| + | *The following table was used to create the mixtures in the cuvette. | ||
| + | **[[Image:Data_Table.JPG]] | ||
| + | **In order to complete trail 2, 150uM and 200uM [adenosine] was run. | ||
| + | **All the listed concentrations were run for trial 3. | ||
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Revision as of 13:36, 13 February 2013
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