User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

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*to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.  
*to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.  
**keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.  
**keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.  
 +
*The following table was used to create the mixtures in the cuvette.
 +
**[[Image:Data_Table.JPG]]
 +
**In order to complete trail 2, 150uM and 200uM [adenosine] was run.
 +
**All the listed concentrations were run for trial 3.
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Revision as of 13:36, 13 February 2013

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+Objective=

  • to finish Trail 2 and run Trial 3 of ADA kinetics

Description

  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Image:Data_Table.JPG
    • In order to complete trail 2, 150uM and 200uM [adenosine] was run.
    • All the listed concentrations were run for trial 3.


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