User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ADA Kinetics</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Revision as of 14:12, 13 February 2013

ADA Kinetics Main project page
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Objective

  • to finish Trail 2 and run Trial 3 of ADA kinetics

Description

  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Image:Data_Table.JPG
    • In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
    • All the listed concentrations were run for trial 3.


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