User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

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|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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==Objective==
==Objective==
*to finish Trail 2 and run Trial 3 of ADA kinetics
*to finish Trail 2 and run Trial 3 of ADA kinetics
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**In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
**In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
**All the listed concentrations were run for trial 3.
**All the listed concentrations were run for trial 3.
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==Data==
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[[Image:Average_Lineweaver-Burk_Plot.png]]
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[[Image:Average_Velocities.png]]
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[[Image:Screen_Shot_2013-02-19_at_11.14.56_AM.png]]
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Revision as of 11:16, 19 February 2013

Search this Project

Customize your entry pages

Objective

  • to finish Trail 2 and run Trial 3 of ADA kinetics

Description

  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Image:Data_Table.JPG
    • In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
    • All the listed concentrations were run for trial 3.

Data

Image:Average_Lineweaver-Burk_Plot.png

Image:Average_Velocities.png

Image:Screen_Shot_2013-02-19_at_11.14.56_AM.png


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