User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/20

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(Aspirin concentration for ADA Kinetic Assay)
Current revision (17:02, 8 May 2013) (view source)
(Data)
 
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==Aspirin concentration for ADA Kinetic Assay==
 
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* The assay for the concentrations below will be conducted next laboratory period.
 
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[[Image:Screen_Shot_2013-02-19_at_1.13.00_PM.png|center]]
 
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==ADA Kinetic Assay for obtaining the zero point==
 
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* The procedure was taken from [[User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20|Mary Mendoza.]]
 
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* The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
 
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* UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
 
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* The assays were prepared according to the data below.
 
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[[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]]
 
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* After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.
 
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* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
 
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* An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm.
 
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* Thus, it was decided to run a full spectrum of adenosine at each given concentration specified above for accurate measurement of  the concentration of adenosine.
 
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==Data==
 
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[[Image:Concen1.png|center]]
 
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[[Image:AveVelo1.png|center]]
 
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[[Image:1adeno.png|center]]
 
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[[Image:Lin1.png|center]]
 
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[[Image:UV2.20.png|center]]
 

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