User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/20: Difference between revisions
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== | ==Aspirin concentration for ADA Kinetic Assay== | ||
* | |||
[[Image:Screen_Shot_2013-02-19_at_1.13.00_PM.png|center]] | |||
==ADA Kinetic Assay for obtaining the zero point== | |||
* The procedure is taken from [[User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20 Mary Mendoza.]] | |||
* The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations. | |||
* UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer. | |||
* The assays were prepared according to the data below. | |||
[[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]] | |||
* After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1. | |||
* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution. | |||
* An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm. | |||
* Thus, it was decided to run a full spectrum of adenosine at each given concentration specified above for accurate measurement of the concentration of adenosine. | |||
==Data== | |||
[[Image:Concen1.png|center]] | |||
[[Image:AveVelo1.png|center]] | |||
[[Image:1adeno.png|center]] | |||
[[Image:Lin1.png|center]] | |||
[[Image:UV2.20.png|center]] | |||
Revision as of 12:25, 22 February 2013
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Aspirin concentration for ADA Kinetic AssayADA Kinetic Assay for obtaining the zero point
Data
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