User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/27

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*More 0.05M phosphate buffer was prepared according to the procedure outlined in [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/01/23| January's entry]].
*More 0.05M phosphate buffer was prepared according to the procedure outlined in [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/01/23| January's entry]].
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==Data==
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[[Image:20130227_ADA_Kinetics_3T.tiff]]
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[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff]]
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[[Image:20130227_average_ADA_Kinetics_3T.tiff]]
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Revision as of 15:52, 27 February 2013

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Objective

  • to begin ADA + Inhibitor kinetics

Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

Image:Screen_Shot_2013-02-27_at_12.43.29_PM.png

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.


Data

Image:20130227_ADA_Kinetics_3T.tiff

Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff

Image:20130227_average_ADA_Kinetics_3T.tiff



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