User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/27: Difference between revisions

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==Objective==
*to begin ADA + Inhibitor kinetics


==Description==
*150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:
[[Image:Screen_Shot_2013-02-27_at_12.43.29_PM.png]]


*25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.


*More 0.05M phosphate buffer was prepared according to the procedure outlined in [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/01/23| January's entry]].


*ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:


[[Image:Screen_Shot_2013-02-27_at_2.57.04_PM.png]]


==Data from [Inhibitor] = 0==
[[Image:20130227_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]]
==Data from [Inhibitor] = 50uM==
[[Image:Screen_Shot_2013-03-05_at_12.03.39_PM.png]]


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