User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/27: Difference between revisions

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==Data from [Inhibitor] = 0==
==Data from [Inhibitor] = 0==


[[Image:20130227_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_ADA_Kinetics_3T.tiff|px100]]


[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff|px100]]


[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_average_ADA_Kinetics_3T.tiff|px100]]


==Data from [Inhibitor] = 50uM==
==Data from [Inhibitor] = 50uM==

Revision as of 10:06, 5 March 2013

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Objective

  • to begin ADA + Inhibitor kinetics

Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.
  • ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:

Data from [Inhibitor] = 0

px100

px100

px100

Data from [Inhibitor] = 50uM


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